HIV infection--induced posttranslational modification of T cell signaling molecules associated with disease progression.
J Clin Invest
; 98(6): 1290-7, 1996 Sep 15.
Article
in En
| MEDLINE
| ID: mdl-8823293
ABSTRACT
In attempt to elucidate the mechanism of the HIV infection induced T cell unresponsiveness, we studied signal-transducing molecules proximal to the T cell receptor (TCR) in T lymphocytes of HIV-infected individuals. Total amounts of protein tyrosine kinases (PTKs) Lck, Fyn, and ZAP-70 and the zeta chain of the TCR were found significantly decreased in T cells of symptomatic/AIDS patients as well as in T cells of individuals in acute and early asymptomatic stages of HIV infection. Unexpectedly, the detection of Lck, Fyn, and ZAP-70 was reversed after the treatment of cell lysates with dithiothreitol. This suggests that PTKs Lck, Fyn, and ZAP-70 were modified by a mechanism altering the status of sulfhydryl groups. Moreover, this mechanism seems to affect selectively T cells of HIV infected patients since B cell PTKs Syk and Lyn were detected structurally and functionally intact. Interestingly, similar alterations of signaling molecules were not detected in T cells of HIV-infected long-term asymptomatic individuals. Modification of T cell PTKs may thus underlie the HIV-induced impairment of lymphocyte function and may potentially predict disease progression.
Full text:
1
Database:
MEDLINE
Main subject:
T-Lymphocytes
/
Signal Transduction
/
HIV Infections
/
Protein Processing, Post-Translational
/
HIV-1
Type of study:
Risk_factors_studies
Limits:
Humans
Language:
En
Journal:
J Clin Invest
Year:
1996
Type:
Article
Affiliation country:
United States