[Exact determination of the lecithin/sphingomyelin ratio in amniotic fluid. Suggestion of a reference methods (author's transl)]. / Determination precise du rapport lecithine/sphingomyeline dans le liquide amniotique: Proposition d'une methode de reference.
Clin Chim Acta
; 79(3): 549-56, 1977 Sep 15.
Article
in Fr
| MEDLINE
| ID: mdl-890988
ABSTRACT
Pulmonary maturity of the fetus can be evaluated by the lecithin/sphingomyelin (L/S) ratio in amniotic fluid. To existing methods of lipid extraction, precipitation with acetone and chromatography, we add a simple and accurate estimation of sphingomyelins (S) and precipitated lecithins (Lp) without acid digestion. The method is reproducible (C.V. less than 9%) for the measurement of Lp/S ratio and gives with accuracy the concentrations of Lp, avoiding possible errors in interpretation of Lp/S. Our results show that at 35 weeks of normal gestation, Lp/S ratio is about 2 and Lp concentration, 10 mg/1.
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Database:
MEDLINE
Main subject:
Phosphatidylcholines
/
Sphingomyelins
/
Amniotic Fluid
Limits:
Humans
Language:
Fr
Journal:
Clin Chim Acta
Year:
1977
Type:
Article