Comparison between immunomagnetic separation, coupled with immunofluorescence, and the techniques of Faust et al. and of Lutz for the diagnosis of Giardia lamblia cysts in human feces
Rev. Inst. Med. Trop. Säo Paulo
; 45(6): 339-342, Nov.-Dec. 2003. ilus, tab
Article
en En
| LILACS
| ID: lil-353986
Biblioteca responsable:
BR1.1
RESUMO
In the present study, the performance of Immunomagnetic Separation technique, coupled with Immunofluorescence (IMS-IFA), was compared with the FAUST et al. and Lutz parasitological techniques for the detection of Giardia lamblia cysts in human feces. One hundred and twenty-seven samples were evaluated by the three techniques at the same time showing a rate of cyst detection of 27.5 percent by IMS-IFA and 15.7 percent by both Faust et al. and Lutz techniques. Data analysis showed a higher sensitivity of IMS-IFA for the detection of G. lamblia cysts in comparison with the techniques of FAUST et al. and Lutz. The use of this methodology as a routine procedure enables the processing of many samples simultaneously, in order to increase recovery rate of G. lamblia cysts and reduce the time of sample storage.
Texto completo:
1
Bases de datos:
LILACS
Asunto principal:
Giardiasis
/
Giardia lamblia
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Rev. Inst. Med. Trop. Säo Paulo
Asunto de la revista:
MEDICINA TROPICAL
Año:
2003
Tipo del documento:
Article
País de afiliación:
Brasil