Validation of new analytical methodology for determining fenoterolhydrobromide by HPLC: application in pharmaceutical products
Rev. Inst. Adolfo Lutz
; 71(2): 355-361, abr.-jun. 2012. tab, graf
Article
en En
| LILACS, SES-SP, SESSP-CTDPROD, SES-SP, SESSP-ACVSES, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: lil-688207
Biblioteca responsable:
BR76.1
ABSTRACT
Fenoterol hydrobromide is a B2-adrenergic agonist agent used for asthma and chronic obstructive pulmonary disease treatment. HPLC methodology was developed and validated for quantitative determination of fenoterol hydrobromide. The methodology was achieved by using a reversed-phase C18column, (150 mm ¡Á 3.9 mm i.d., 5 ¦Ìm) Thermo. The mobile phase was consisted of acetonitrile water(3070, v/v) with 0,1% triethylamine, pH adjusted to 5.0 with formic acid and flow rate of 1.0 mL.min-1with UV detection at 276 nm. The concentration range was from 0.025 to 0.15 mg.mL-1, and the correlation coefficient of analytical curve was >0.999. The detection limit and the quantifying limit (QL) were 0.003mg.mL-1 and 0.012 mg.mL-1, respectively. Intra- and interday relative standard deviations were ¡Ü2.0%. Themetho dology accuracy showed the percentage mean of 99.53%. The described technique was found to be simple, rapid, precise, accurate and sensitive; the advantages over the others current methodologies arethe low-cost and low-polluting conditions. Owing to its simplicity and reliable results, this methodology is suitable to be used in quality control of pharmaceutical drugs containing fenoterol hydrobromide as active componente.
Palabras clave
Texto completo:
1
Bases de datos:
LILACS
Asunto principal:
Hyoscyaminum Bromatum
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Cromatografía Liquida
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Insumos Farmacéuticos
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Prueba de Esfuerzo
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Fenoterol
Idioma:
En
Revista:
Rev. Inst. Adolfo Lutz
Asunto de la revista:
MICROBIOLOGIA
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SAUDE PUBLICA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Brasil