Evaluation of a recombinant rhoptry protein 2 enzyme-linked immunoassay for the diagnosis of toxoplasmosis acquired during pregnancy
Capobiango, Jaqueline Dario; Pagliari, Sthefany; Pasquali, Aline Kuhn Sbruzzi; Nino, Beatriz; Ferreira, Fernanda Pinto; Monica, Thaís Cabral; Tschurtschenthaler, Nely Norder; Navarro, Italmar Teodorico; Garcia, João Luis; Mitsuka-Breganó, Regina; Reiche, Edna Maria Vissoci.
Mem. Inst. Oswaldo Cruz
; 110(6): 732-738, Sept. 2015. tab, graf
Artículo
en Inglés
| LILACS | ID: lil-763098
The aim of this study was to evaluate an enzyme-linked immunoassay with recombinant rhoptry protein 2 (ELISA-rROP2) for its ability to detectToxoplasma gondii ROP2-specific IgG in samples from pregnant women. The study included 236 samples that were divided into groups according to serological screening profiles for toxoplasmosis unexposed (n = 65), probable acute infection (n = 48), possible acute infection (n = 58) and exposed to the parasite (n = 65). When an indirect immunofluorescence assay forT. gondii-specific IgG was considered as a reference test, the ELISA-rROP2 had a sensitivity of 61.8%, specificity of 62.8%, predictive positive value of 76.6% and predictive negative value of 45.4% (p = 0.0002). The ELISA-rROP2 reacted with 62.5% of the samples from pregnant women with probable acute infection and 40% of the samples from pregnant women with previous exposure (p = 0.0180). Seropositivity was observed in 50/57 (87.7%) pregnant women with possible infection. The results underscored that T. gondii rROP2 is recognised by specific IgG antibodies in both the acute and chronic phases of toxoplasmosis acquired during pregnancy. However, the sensitivity of the ELISA-rROP2 was higher in the pregnant women with probable and possible acute infections and IgM reactivity.
Asunto(s)
Femenino Humanos Embarazo Antígenos de Protozoos/inmunología Ensayo de Inmunoadsorción Enzimática/métodos Inmunoglobulina G/sangre Proteínas de la Membrana/inmunología Complicaciones Parasitarias del Embarazo/diagnóstico Proteínas Protozoarias/inmunología Toxoplasmosis/diagnóstico Antígenos de Protozoos/sangre Intervalos de Confianza Ensayo de Inmunoadsorción Enzimática/normas Técnica del Anticuerpo Fluorescente Indirecta Inmunoglobulina G/aislamiento & purificación Inmunoglobulina M/sangre Inmunoglobulina M/aislamiento & purificación Invenciones/normas Proteínas de la Membrana/genética Valor Predictivo de las Pruebas Complicaciones Parasitarias del Embarazo/sangre Complicaciones Parasitarias del Embarazo/inmunología Proteínas Protozoarias/genética Proteínas Recombinantes Estándares de Referencia Sensibilidad y Especificidad Toxoplasma/inmunología Toxoplasmosis/sangre Toxoplasmosis/inmunología
Biblioteca responsable:
BR1.1
powered by iAHx-2.18-89
Portal de Búsqueda de la BVS Enfermería