Proteolytic fragments of Alzheimer's disease-associated presenilin 1 are present in synaptic organelles and growth cone membranes of rat brain.
J Neurochem
; 72(4): 1564-73, 1999 Apr.
Article
en En
| MEDLINE
| ID: mdl-10098862
ABSTRACT
Previous studies have demonstrated the molecular linkage of three causative genes for early-onset Alzheimer's disease the presenilin 1 gene on chromosome 14, the presenilin 2 gene on chromosome 1, and the amyloid precursor protein gene on chromosome 21. In the present study, we have investigated the distributions of the approximately 20-kDa C-terminal and approximately 30-kDa N-terminal fragments of presenilin 1 and the amyloid precursor protein in rat brain and compared them with the distribution of several marker proteins. The fragments of presenilin 1 are present in synaptic plasma membranes, neurite growth cone membranes, and small synaptic vesicles of rat brain. Both proteolytic fragments are coenriched in the corresponding tissue fractions. Based on this observation, it seems likely that N- and C-terminal presenilin 1 fragments form a functional unit while remaining associated. In contrast to a predominant subcellular localization of presenilin 1 to the endoplasmic reticulum and Golgi apparatus in different cell lines, our results indicate that rat brain presenilin 1 fragments exit from these biosynthetic compartments to reach synaptic organelles in neurons.
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Bases de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Sinapsis
/
Conos de Crecimiento
/
Enfermedad de Alzheimer
/
Proteínas de la Membrana
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
Idioma:
En
Revista:
J Neurochem
Año:
1999
Tipo del documento:
Article
País de afiliación:
Alemania