Global expression profiling of yeast treated with an inhibitor of amino acid biosynthesis, sulfometuron methyl.
Physiol Genomics
; 3(2): 83-92, 2000 Aug 09.
Article
en En
| MEDLINE
| ID: mdl-11015603
ABSTRACT
The expression pattern of 1,529 yeast genes in response to sulfometuron methyl (SM) was analyzed by DNA microarray technology. SM, a potent herbicide, inhibits acetolactate synthase, a branched-chain amino acid biosynthetic enzyme. Exposure of yeast cells to 0.2 microg/ml SM resulted in 40% growth inhibition, a Gcn4p-mediated induction of genes involved in amino acid and cofactor biosynthesis, and starvation response. The accumulation of intermediates led to the induction of stress response genes and the repression of genes involved in carbohydrate metabolism, nucleotide biosynthesis, and sulfur assimilation. Extended exposure to SM led to a relaxation of the initial response and induction of sugar transporter and ergosterol biosynthetic genes, as well as repression of histone and lipid metabolic genes. Exposure to 5 microg/ml SM resulted in >98% growth inhibition and stimulated a similar initial expression change, but with no relaxation after extended exposure. Instead, more stress response and DNA damage repair genes become induced, suggesting a serious cellular consequence. Other salient features of metabolic regulation, such as the coordinated expression of cofactor biosynthetic genes with amino acid biosynthetic ones, were evident from our data. A potential link between SM sensitivity and ergosterol metabolism was uncovered by expression profiling and confirmed by genetic analysis.
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Bases de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Compuestos de Sulfonilurea
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Perfilación de la Expresión Génica
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Proteínas de Saccharomyces cerevisiae
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Proteínas de Unión al ADN
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Genes Fúngicos
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Aminoácidos
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Herbicidas
Idioma:
En
Revista:
Physiol Genomics
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2000
Tipo del documento:
Article
País de afiliación:
Estados Unidos