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Improved method for pepsinolysis of mouse IgG(1) molecules to F(ab')(2) fragments.
Wilson, David S; Wu, Jiangchun; Peluso, Paul; Nock, Steffen.
Afiliación
  • Wilson DS; Department of Biochemistry, Zyomyx, Inc., 26101 Research Road, Hayward, CA 94545, USA.
J Immunol Methods ; 260(1-2): 29-36, 2002 Feb 01.
Article en En | MEDLINE | ID: mdl-11792373
Pepsinolysis of immunoglobulin (IgG) to yield F(ab')(2) fragments has been utilized for over 40 years, but the most common subclass of mouse immunoglobulin, IgG(1), is resistant to pepsin cleavage. We show here that this resistance is due to N-linked glycosylation. Deglycosylation by peptide: N-glycosidase F (PNGase F) improves pepsinolysis to generate F(ab')(2) fragments for mouse and rat monoclonal IgG(1) and, in some cases, IgG(2b). This effect was observed for antibodies derived from tissue cell culture or ascites fluid, as well as for polyclonal IgGs from mouse serum. As a result of this finding, the preparation of F(ab')(2) fragments from nearly all mouse IgG molecules should now be a robust procedure.
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Bases de datos: MEDLINE Asunto principal: Inmunoglobulina G / Fragmentos Fab de Inmunoglobulinas Límite: Animals Idioma: En Revista: J Immunol Methods Año: 2002 Tipo del documento: Article País de afiliación: Estados Unidos
Buscar en Google
Bases de datos: MEDLINE Asunto principal: Inmunoglobulina G / Fragmentos Fab de Inmunoglobulinas Límite: Animals Idioma: En Revista: J Immunol Methods Año: 2002 Tipo del documento: Article País de afiliación: Estados Unidos