Enzyme-based sensor arrays for rapid characterization of complex disaccharide solutions.
Anal Biochem
; 303(1): 42-8, 2002 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-11906149
ABSTRACT
An enzyme-based sensor array has been developed to detect multiple disaccharides in aqueous solutions. Porous agarose beads, derivatized with enzymes for assaying disaccharides, are localized within wells etched into a silicon chip in a regular 5 x 7 array. Each well is individually addressable and acts as a microanalysis chamber where sample solution passes through the agarose matrix and is exposed to the enzymes. Detection is achieved by observing the increase in absorbance of a quinoneimine dye produced during the reaction. This technique is used to quantify the disaccharides lactose, sucrose, and maltose and the monosaccharide glucose. Preexisting glucose in the sample complicates multicomponent sensing but can be accounted for by including a glucose sensor in the array. This detection strategy is applied to the simultaneous analysis of these sugars in several beverages.
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Bases de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Disacáridos
/
Glucosa
/
Glucosa Oxidasa
/
Peroxidasa de Rábano Silvestre
Idioma:
En
Revista:
Anal Biochem
Año:
2002
Tipo del documento:
Article
País de afiliación:
Estados Unidos