Simultaneous analysis of gamma-hydroxybutyric acid, gamma-butyrolactone, and 1,4-butanediol by micellar electrokinetic chromatography.
Forensic Sci Int
; 125(2-3): 113-9, 2002 Feb 18.
Article
en En
| MEDLINE
| ID: mdl-11909651
ABSTRACT
A micellar electrokinetic chromatography (MEKC) method was optimised for simultaneous analysis of gamma-hydroxybutyric acid (GHB), gamma-butyrolactone (GBL), and 1,4-butanediol (BD). Best conditions for separation and baseline stability were achieved using a carrier electrolyte comprising 30.0mM sodium barbital and 150.0mM sodium dodecyl sulphate (SDS) at pH 10.2. Calibration functions were linear, giving correlation coefficients (r(2)) >0.998 for the three target compounds. Limits of detection (LOD) defined as three times the noise, were 5.1mg/l, 0.34 and 0.25g/l for GHB, GBL and BD, respectively. The repeatability of migration times and peak areas, expressed as the R.S.D. (n = 9) was better than 0.41 and 3.05%, respectively. Some casework samples were analysed using the optimised conditions.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Solventes
/
Butileno Glicoles
/
4-Butirolactona
/
Cromatografía Capilar Electrocinética Micelar
/
Hidroxibutiratos
Idioma:
En
Revista:
Forensic Sci Int
Año:
2002
Tipo del documento:
Article
País de afiliación:
Suecia