Prediction of the structure of human Janus kinase 2 (JAK2) comprising JAK homology domains 1 through 7.
Protein Eng
; 15(9): 727-37, 2002 Sep.
Article
en En
| MEDLINE
| ID: mdl-12456871
ABSTRACT
A theoretical model of human Janus kinase 2 (JAK2) comprising all seven Janus homology domains is presented. The model was generated by application of homology modelling approaches. The three-dimensional structure contains, starting from the N-terminus, FERM (4.1, ezrin, radixin, moesin), SH2 (Src homology region 2), tyrosine kinase-like, and tyrosine kinase domains. The predicted inter-domain orientation in JAK2 is discussed and the currently existing mutational data for Janus kinases are evaluated. Structural details of the SH2 and the FERM domains are presented. The predictions indicate that the SH2 domain is not fully functional. A number of hydrophobic amino acids of the FERM domain that are predicted to be involved in the constitutive association with the cytokine receptors are highlighted. The model gives new insights into the structure-function relationship of this important protein, and areas that could be investigated by mutation studies are highlighted.
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Bases de datos:
MEDLINE
Asunto principal:
Proteínas Tirosina Quinasas
/
Proteínas Proto-Oncogénicas
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Protein Eng
Asunto de la revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Año:
2002
Tipo del documento:
Article
País de afiliación:
Reino Unido