New HLA-DRB1*07 allele identified from microlymphocytotoxicity/SSP discrepancies.
Tissue Antigens
; 60(6): 539-41, 2002 Dec.
Article
en En
| MEDLINE
| ID: mdl-12542749
ABSTRACT
A HLA-DRB1*07 variant allele has been identified in a cadaver kidney donor. Serological typing using monoclonal antibodies detected HLA-DR4 and HLA-DR7. HLA class II DNA typing using sequence-specific primer (PCR-SSP) polymerase chain reaction only detected DRB1*04, while sequence-specific oligonucleotide (PCR-SSO) polymerase chain reaction confirmed the presence of both DRB1*04 and DRB1*07 alleles, although two extra reactions were also found. Exon 2 of the HLA-DRB1*07 was isolated using allele-specific PCR, then cloned and sequenced. Four mutations, at positions 170 (T --> C), 171 (C --> T), 174 (C --> G), and 179 (C --> A), were observed. These mutations changed codons 57 and 60 (V --> A; S --> Y, respectively). This amino acid sequence at position 56-61 is only found in DRB1*0811.
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Bases de datos:
MEDLINE
Asunto principal:
Antígenos HLA-DR
Límite:
Humans
Idioma:
En
Revista:
Tissue Antigens
Año:
2002
Tipo del documento:
Article
País de afiliación:
Francia