Ribonuclease is partly responsible for the HIV-1 inhibitory effect activated by HLA alloantigen recognition.
AIDS
; 17(4): 481-6, 2003 Mar 07.
Article
en En
| MEDLINE
| ID: mdl-12598767
ABSTRACT
OBJECTIVE:
This study was performed to determine whether ribonucleases (RNases) contribute to the soluble HIV-1 inhibitory activity that results from the recognition of HLA alloantigens. DESIGN ANDMETHODS:
Supernatants from mixed lymphocyte reactions of peripheral blood mononuclear cells from healthy HLA-discordant individuals exhibited HIV-1 inhibitory activity (alloantigen-stimulated factors; ASF). These supernatants were tested for their sensitivity to heating (90 degrees C for 3 min), and for the presence of three RNases belonging to the RNase A superfamily eosinophil-derived neurotoxin (EDN); RNase A; and angiogenin. Polyclonal antibodies specific for these RNases were used for Western blot analysis of the ASF, as well as for blocking the HIV-1 inhibitory activity of ASF. In addition, an RNase inhibitor (RI) was used to determine whether the anti-viral activity of ASF was due to RNase activity.RESULTS:
HIV-1 inhibitory activity of ASF was (i). resistant to heat treatment; (ii). blocked by 58% with an antibody specific for EDN, but not with antibodies against RNase A or angiogenin; and (iii) blocked by 65-100% with an RI. Moreover, Western blot analysis with an anti-EDN antibody detected EDN in the ASF.CONCLUSION:
These findings indicate that the majority of the soluble HIV-1 inhibitory activity contained in the supernatants of mixed lymphocyte reactions is due to EDN or a closely related RNase.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Antivirales
/
Ribonucleasas
/
Infecciones por VIH
/
VIH-1
/
Antígenos de Histocompatibilidad
Límite:
Humans
Idioma:
En
Revista:
AIDS
Asunto de la revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos