Surprisingly fast disappearance of beta-lactam selection pressure in cultivation as detected with novel biosensing approaches.
J Microbiol Methods
; 53(1): 37-42, 2003 Apr.
Article
en En
| MEDLINE
| ID: mdl-12609721
ABSTRACT
Tetracycline and beta-lactam resistances among others are used as selection markers in the production of recombinant proteins. The beta-lactam resistance is based on degradation, i.e. the selection pressure gradually disappears from the culture, whereas tetracycline resistance is based on active efflux. We have studied the kinetics of the stability of antibiotic selection pressure in culture using a simple model system (pBR322 in Escherichia coli). Concentrations of ampicillin, carbenicillin and tetracycline were measured with novel sensor cells developed in our lab. These cells are specifically induced to produce light in the presence of the drugs and here their performance was shown to be excellent in monitoring antibiotic concentrations in cell culture. The sensor cells are cheap to produce and use and a high number of samples can be analysed simultaneously. To our surprise, ampicillin and carbenicillin were completely degraded after 2.5-3.0 h of culture, although it has been widely claimed that especially carbenicillin is a good selective agent, whereas tetracycline was stable in culture. beta-lactamase activity in culture was found to correlate with the kinetics of ampicillin degradation.
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Bases de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Resistencia betalactámica
/
Beta-Lactamas
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Microbiol Methods
Año:
2003
Tipo del documento:
Article
País de afiliación:
Finlandia