Possible contribution of apoptosis-inducing factor (AIF) and reactive oxygen species (ROS) to UVB-induced caspase-independent cell death in the T cell line Jurkat.
J Leukoc Biol
; 73(3): 399-406, 2003 Mar.
Article
en En
| MEDLINE
| ID: mdl-12629154
ABSTRACT
We analyzed the mechanism of UVB-induced cell death using the Jurkat T cell line. Apoptosis was assessed by measuring phosphatidylserine (PS) externalization, caspase activity, the decrease in mitochondrial membrane potential (Delta Psi m), nucleosomal DNA fragmentation, and morphological changes such as chromatin condensation. The mitochondrio-nuclear translocation of apoptosis-inducing factor (AIF) was evaluated by confocal laser microscopy. The cell death pattern of UVB-irradiated cells was similar to the Fas-induced cell death pattern. However, zVAD-fmk inhibited the nucleosomal fragmentation of DNA but not the externalization of PS, decrease in Delta Psi m, or mitochondrio-nuclear translocation of AIF. N-acetyl L-cysteine significantly inhibited the translocation of AIF induced by UVB. These results suggested that caspase-dependent and -independent pathways were involved in UVB-induced cell death in Jurkat cells, and the mitochondrio-nuclear translocation of AIF was associated with the latter pathway. In addition, reactive oxygen species generated by UVB might be involved in inducing the mitochondrio-nuclear translocation of AIF.
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Bases de datos:
MEDLINE
Asunto principal:
Rayos Ultravioleta
/
Linfocitos T
/
Muerte Celular
/
Especies Reactivas de Oxígeno
/
Flavoproteínas
/
Proteínas de la Membrana
Límite:
Humans
Idioma:
En
Revista:
J Leukoc Biol
Año:
2003
Tipo del documento:
Article
País de afiliación:
Japón