Local synthesis of the rat Vasopressin precursor in dendrites of in vitro cultured nerve cells.
Brain Res Mol Brain Res
; 114(2): 115-22, 2003 Jun 10.
Article
en En
| MEDLINE
| ID: mdl-12829321
ABSTRACT
Vasopressin (VP) mRNA is subject to dendritic targeting both in vivo and in primary cultured neurons microinjected with an appropriate expression vector. We have constructed a vector encoding the mutant Brattleboro rat VP precursor which is non-diffusable, because it cannot leave the site of its synthesis, the rough endoplasmic reticulum. Expression of this construct in cultured nerve cells shows that the mutant protein is readily detectable in dendrites when mRNA transport has occurred, while dendrites devoid of the mRNA lack the protein. These results demonstrate that neurons have the capacity to locally synthesize secretory proteins in the dendritic compartment.
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Bases de datos:
MEDLINE
Asunto principal:
Precursores de Proteínas
/
Vasopresinas
/
Compartimento Celular
/
Ganglio Cervical Superior
/
Dendritas
Límite:
Animals
Idioma:
En
Revista:
Brain Res Mol Brain Res
Asunto de la revista:
BIOLOGIA MOLECULAR
/
CEREBRO
Año:
2003
Tipo del documento:
Article
País de afiliación:
Alemania