[The first cholera case diagnosed early in the clinical laboratory by DNA probe method].
Kansenshogaku Zasshi
; 66(12): 1645-50, 1992 Dec.
Article
en Ja
| MEDLINE
| ID: mdl-1294670
ABSTRACT
An alkaline-phosphatase-labelled synthetic oligonucleotide probe was applied to detect the structural gene of cholera enterotoxin (ctx). This DNA probe has a complementary base sequence to 30 base of the CT-A subunit. This method was, for the first time, applied to diagnosis of a diarrheal patient. The probe detected ctx rapidly and simply as compared with reversed passive latex agglutination (RPLA) and Beads-ELISA. The cfu minimal dose for detection with the probe was about 10(6-7)/ml. This method can be easily performed in any clinical laboratory because the procedure is safe, simple and rapid (it can be completed within about 3 hours).
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Bases de datos:
MEDLINE
Asunto principal:
Sondas de ADN
/
Cólera
Tipo de estudio:
Diagnostic_studies
Límite:
Adult
/
Humans
/
Male
País/Región como asunto:
Asia
Idioma:
Ja
Revista:
Kansenshogaku Zasshi
Año:
1992
Tipo del documento:
Article