Telomere-associated chromosome fragmentation: applications in genome manipulation and analysis.
Nat Genet
; 2(4): 275-82, 1992 Dec.
Article
en En
| MEDLINE
| ID: mdl-1303279
ABSTRACT
Telomere-associated chromosome fragmentation (TACF) is a new approach for chromosome mapping based on the non-targeted introduction of cloned telomeres into mammalian cells. TACF has been used to generate a panel of somatic cell hybrids with nested terminal deletions of the long arm of the human X chromosome, extending from Xq26 to the centromere. This panel has been characterized using a series of X chromosome loci. Recovery of the end clones by plasmid rescue produces a telomeric marker for each cell line and partial sequencing will allow the generation of sequence tagged sites (STSs). TACF provides a powerful and widely applicable method for genome analysis, a general way of manipulating mammalian chromosomes and a first step towards constructing artificial mammalian chromosomes.
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Bases de datos:
MEDLINE
Asunto principal:
Cromosoma X
/
Mapeo Cromosómico
/
Telómero
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Genet
Asunto de la revista:
GENETICA MEDICA
Año:
1992
Tipo del documento:
Article
País de afiliación:
Reino Unido