Electrically controlled proliferation of human carcinoma cells cultured on the surface of an electrode.
J Biotechnol
; 18(1-2): 129-39, 1991 Apr.
Article
en En
| MEDLINE
| ID: mdl-1367098
ABSTRACT
Human carcinoma cells, MKN45, were cultured on the surface of a metal-coated plastic plate electrode the potential of which was controlled. The proliferation rate and cell morphology were altered depending on the applied potential. Cell proliferation was halted in the potential range above 0.4 V vs. Ag/AgCl, although cells started to proliferate again when the applied potential was shifted from 0.4 V to 0.1 V vs. Ag/AgCl. Fluorescence probe studies indicated that the fluidity of plasma membrane decreased in association with halting of cell proliferation. These results suggest that electrical stimulation causes cells to temporarily halt proliferation, and that cell proliferation was reversibly controlled by electrode potential. The mechanism is interpreted in relation to the change of plasma membrane structure represented by membrane fluidity.
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Bases de datos:
MEDLINE
Asunto principal:
Células Tumorales Cultivadas
Límite:
Humans
Idioma:
En
Revista:
J Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
Año:
1991
Tipo del documento:
Article
País de afiliación:
Japón