On/off switch mediated by Exo+ polymerases: experimental analysis for its physiological and technological implications.
J Biochem Mol Biol
; 36(6): 529-32, 2003 Nov 30.
Article
en En
| MEDLINE
| ID: mdl-14659069
ABSTRACT
The potential physiological role and technological application of the premature termination of DNA polymerization through the off-switch of exo+ polymerases were studied using 3' phosphorothioate-modified or unmodified primers with single base mismatch distal to the 3' terminus. With exonuclease-digestible unmodified primers, a gradient premature termination of DNA polymerization was observed when amplified with exo+ polymerases. With 3' allele specific phosphorothioate-modified primers, an efficient off-switch effect occurred in the discrimination of a single nucleotide polymorphism when directly using genomic DNA. Clearly, the off-switch of exo+ polymerases is useful in biomedical research.
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Bases de datos:
MEDLINE
Asunto principal:
ADN Polimerasa Dirigida por ADN
Idioma:
En
Revista:
J Biochem Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
2003
Tipo del documento:
Article
País de afiliación:
China