Broadly cross-reactive HIV neutralizing human monoclonal antibody Fab selected by sequential antigen panning of a phage display library.
J Immunol Methods
; 283(1-2): 17-25, 2003 Dec.
Article
en En
| MEDLINE
| ID: mdl-14659896
ABSTRACT
Identification of broadly cross-reactive human monoclonal antibodies (mAbs) has major implications for development of vaccines, inhibitors and research tools. Here we describe a sequential antigen panning (SAP) methodology that may facilitate the selection of such antibodies. An HIV-specific antibody Fab (m18) was selected from a human Fab phage-display library by SAP against several recombinant soluble HIV envelope glycoproteins (Envs) and Env-sCD4 complexes. This Fab bound to a variety of recombinant soluble Envs (gp140s) from primary HIV isolates representing different clades, and inhibited cell fusion and virus entry mediated by Envs of primary HIV isolates. The methodology and the results may have implications for development of HIV vaccines and inhibitors, as well as for identification of antibodies to conserved epitopes on rapidly mutating viruses and cells.
Buscar en Google
Bases de datos:
MEDLINE
Asunto principal:
Fragmentos Fab de Inmunoglobulinas
/
Anticuerpos Anti-VIH
/
Biblioteca de Péptidos
/
Anticuerpos Monoclonales
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
2003
Tipo del documento:
Article
País de afiliación:
Estados Unidos