gp91phox-dependent expression of platelet CD40 ligand.
Circulation
; 110(10): 1326-9, 2004 Sep 07.
Article
en En
| MEDLINE
| ID: mdl-15249506
ABSTRACT
BACKGROUND:
CD40 ligand (CD40L) expression on platelets is mediated by agonists, but the underlying mechanism is still unclear. METHODS ANDRESULTS:
CD40L expression was measured in platelets from healthy subjects both with and without the addition of antioxidants or a phospholipase A2 (PLA2) inhibitor and in platelets from 2 patients with an inherited deficiency of gp91phox. Immunoprecipitation analysis was also performed to determine whether normal platelets showed gp91phox expression. Unlike catalase and mannitol, superoxide dismutase inhibited agonist-induced platelet CD40L expression in healthy subjects. Immunoprecipitation analysis also showed that platelets from healthy subjects expressed gp91phox. In 2 male patients with inherited gp91phox deficiency, collagen-, thrombin-, and arachidonic acid-stimulated platelets showed an almost complete absence of superoxide anion (O(2)(-)) and CD40L expression. Incubation of platelets from healthy subjects with a PLA2 inhibitor almost completely prevented agonist-induced O(2)(-) and CD40L expression.CONCLUSIONS:
These data provide the first evidence that platelet CD40L expression occurs via arachidonic acid-mediated gp91phox activation.
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Bases de datos:
MEDLINE
Asunto principal:
Plaquetas
/
Glicoproteínas de Membrana
/
Ácido Araquidónico
/
Especies Reactivas de Oxígeno
/
NADPH Oxidasas
/
Ligando de CD40
Límite:
Adult
/
Humans
/
Male
Idioma:
En
Revista:
Circulation
Año:
2004
Tipo del documento:
Article
País de afiliación:
Italia