Phagocytic cells of the thymic reticulum interact with thymocytes via extracellular matrix ligands and receptors.
Cell Immunol
; 229(1): 21-30, 2004 May.
Article
en En
| MEDLINE
| ID: mdl-15331325
ABSTRACT
We previously showed that, in the context of thymic epithelial cells, thymocyte migration is partially controlled by extracellular matrix (ECM)-mediated interactions. Herein we evaluated whether these interactions could be involved in cell migration related events in the context of non-epithelial cells of the thymic microenvironment, the phagocytic cells of the thymic reticulum (PTR). We first showed, by immunocytochemistry, cytofluorometry, and RT-PCR, that PTR produce ECM components, including fibronectin and laminin, and express the corresponding integrin-type receptors, VLA-4, VLA-5, and VLA-6. Thymocytes adhere onto PTR monolayers, with immature CD4(+)CD8(+) cells being predominant. Importantly, such an adhesion is partially mediated by ECM ligands and receptors, since it was impaired by anti-ECM or anti-ECM receptor antibodies. Conjointly, our data reveal that the ECM-dependence for thymocyte adhesion onto the thymic microenvironment is not restricted to the epithelial cells, being also seen when they encounter non-epithelial phagocytic cells.
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Bases de datos:
MEDLINE
Asunto principal:
Fagocitos
/
Timo
/
Comunicación Celular
/
Matriz Extracelular
Límite:
Animals
Idioma:
En
Revista:
Cell Immunol
Año:
2004
Tipo del documento:
Article
País de afiliación:
Brasil