[Assesment of genetic diversity of allelopathic rice germplasm based on RAPD and ISSR].

ABSTRACT

Random Amplified Polymorphic DNA (RAPD) and Inter-simple Sequence Repeat (ISSR) methods were used to detect the genetic diversity of 57 allelopathic rice accessions which were introduced from 10 countries or areas. A total of 12 RAPD primers and seven ISSR primers were indentified with polymorphism among the entries. For RAPD markers, 85 polymorphic bands were produced, percentage of polymorphic bands (PPB) were 69.4%. For ISSR marker, 34 polymorphic bands were generated, PPB were 53.0%. The result from the clustering analysis by UPGMA indicated that those accessions from the same geographical location were clustered into one group. It was also found that some rice accessions with higher allelopathic potential were clustered together, implying that the genes conferring allelopathy in those rice accessions might be isolocus. However, some rice accessions with different allelopathic potential clustered into the same group performed lower level of generic polymorphism which was attributed to oriented selection for other traits in breeding program. The estimates of correlation coefficient of RAPD and ISSR based on the genetic similarity matrices were significantly correlated.