Detection of bladder carcinoma by combined testing of urine for hyaluronidase and cytokeratin 20 RNAs.
Cancer
; 103(7): 1356-62, 2005 Apr 01.
Article
en En
| MEDLINE
| ID: mdl-15717321
ABSTRACT
BACKGROUND:
A new, sensitive, noninvasive method for the detection of urothelial carcinomas of the urinary bladder would open new possibilities in both the diagnosis and followup of patients.METHODS:
This study included 228 patients diagnosed with bladder carcinoma, 68 patients with benign bladder lesions, and 44 healthy persons served as the control group. All were subjected to serologic schistosomiasis antibody assay in serum, urine cytology, estimation of urine hyaluronic acid (HA) by enzyme-linked immunosorbent assay, and detection of CK-20 and hyaluronidase (HAase) by reverse transcription polymerase chain reaction (RT-PCR) in urothelial cells from voided urine.RESULTS:
HA mean rank was higher in benign and malignant groups than in the healthy group (P < 0.0001) and was significantly related to tumor grade (P = 0.021). HA best-cutoff, determined using receiver operating characteristic curve to discriminate between malignant and nonmalignant groups, was 58.5 units/mg protein at 85.8% sensitivity and 60.7% specificity. HAase RNA showed superior sensitivity (90.8%) over cytology (68.9%) and CK-20 (78.1%) with specificity of 93.4%, 98.1% and 80.2%, respectively. The sensitivity reached 94.7% at a specificity of 91.5% when combined with CK-20. All 4 of the investigated markers were related to grade at P <0.05. Whereas only HAase and CK-20 were significantly related to stage (P < 0.05). As to schistosomiasis, only HAase RNA positivity was significantly associated (P = 0.038).CONCLUSIONS:
HAase RNA is a promising noninvasive test with high sensitivity and specificity in bladder carcinoma detection.
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Bases de datos:
MEDLINE
Asunto principal:
Neoplasias de la Vejiga Urinaria
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Hialuronoglucosaminidasa
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Proteínas de Filamentos Intermediarios
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
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Observational_studies
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Risk_factors_studies
Límite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Cancer
Año:
2005
Tipo del documento:
Article
País de afiliación:
Egipto