Methodological issues affecting the determination of plasma matrix metalloproteinase (MMP)-2 and MMP-9 activities.
Clin Biochem
; 38(5): 410-4, 2005 May.
Article
en En
| MEDLINE
| ID: mdl-15820769
ABSTRACT
OBJECTIVES:
To examine whether a linear relationship exists between the volume of plasma loaded in each gel lane and the gelatinolytic activities of plasma matrix metalloproteinase (pMMP)-2 and pMMP-9, and the influences of plasma storage temperature, fasting, and a variable number of repeated freeze-thaw cycles. DESIGN ANDMETHODS:
Plasma from venous blood collected into EDTA tubes was stored for 1 month at -20 degrees C or at -70 degrees C until assayed for gelatin zymography of pMMP-2 and pMMP-9. The effects of food intake, repeated freeze-thaw cycles, and the gelatinolytic activity produced by serial dilutions of plasma were examined.RESULTS:
Storage temperature, fasting, and up to five repeated freeze-thaw cycles had no effect on the activities of pMMPs (all P < 0.05). A linear relationship was found with plasma volumes below 0.75 microL/lane for MMP-2, and from 0.75 microL/lane to 2 microL/lane for MMP-9.CONCLUSIONS:
Different volumes of plasma should be studied when pMMP-2 and pMMP-9 activities are studied. Storage temperature, repeated freeze-thaw cycles, and food intake do not affect the activities of pMMPs.
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Bases de datos:
MEDLINE
Asunto principal:
Metaloproteinasa 2 de la Matriz
/
Metaloproteinasa 9 de la Matriz
Límite:
Humans
Idioma:
En
Revista:
Clin Biochem
Año:
2005
Tipo del documento:
Article
País de afiliación:
Brasil