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Mutant PIK3CA promotes cell growth and invasion of human cancer cells.
Samuels, Yardena; Diaz, Luis A; Schmidt-Kittler, Oleg; Cummins, Jordan M; Delong, Laura; Cheong, Ian; Rago, Carlo; Huso, David L; Lengauer, Christoph; Kinzler, Kenneth W; Vogelstein, Bert; Velculescu, Victor E.
Afiliación
  • Samuels Y; The Sidney Kimmel Comprehensive Cancer Center and The Howard Hughes Medical Institute, The Johns Hopkins University Medical Institutions, Baltimore, MD 21231, USA.
Cancer Cell ; 7(6): 561-73, 2005 Jun.
Article en En | MEDLINE | ID: mdl-15950905
ABSTRACT
PIK3CA is mutated in diverse human cancers, but the functional effects of these mutations have not been defined. To evaluate the consequences of PIK3CA alterations, the two most common mutations were inactivated by gene targeting in colorectal cancer (CRC) cells. Biochemical analyses of these cells showed that mutant PIK3CA selectively regulated the phosphorylation of AKT and the forkhead transcription factors FKHR and FKHRL1. PIK3CA mutations had little effect on growth under standard conditions, but reduced cellular dependence on growth factors. PIK3CA mutations resulted in attenuation of apoptosis and facilitated tumor invasion. Treatment with the PI3K inhibitor LY294002 abrogated PIK3CA signaling and preferentially inhibited growth of PIK3CA mutant cells. These data have important implications for therapy of cancers harboring PIK3CA alterations.
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Bases de datos: MEDLINE Asunto principal: Fosfatidilinositol 3-Quinasas / Proliferación Celular / Invasividad Neoplásica Tipo de estudio: Prognostic_studies Idioma: En Revista: Cancer Cell Asunto de la revista: NEOPLASIAS Año: 2005 Tipo del documento: Article País de afiliación: Estados Unidos
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Bases de datos: MEDLINE Asunto principal: Fosfatidilinositol 3-Quinasas / Proliferación Celular / Invasividad Neoplásica Tipo de estudio: Prognostic_studies Idioma: En Revista: Cancer Cell Asunto de la revista: NEOPLASIAS Año: 2005 Tipo del documento: Article País de afiliación: Estados Unidos