[Physiological comportment and in vivo sensitivity of Sporothrix schenckii isolates maintained for 18 years by two preservation methods]. / Comportamiento fisiológico y de sensibilidad in vitro de aislamientos de Sporothrix schenckii mantenidos 18 años por dos métodos de preservación.
Rev Iberoam Micol
; 22(3): 151-6, 2005 Sep.
Article
en Es
| MEDLINE
| ID: mdl-16309350
ABSTRACT
We compared two methods for the preservation of fungi, Castellani's method and repeated passage in Sabouraud medium-agar, on five isolates of Sporothrix schenckii that were preserved for 18 years at room temperature by both procedures. They were evaluated for viability of the strains, growth rate, morphological and physiological characteristics, and in vitro sensitivity to iodide, itraconazole, terbinafine and posaconazole. 100% viability was observed in all of the isolates, with slower growth rate on strains preserved in water compared to strains periodically re-cultured. The typical morphological feature of these fungi was preserved by both methodologies. With regard to enzymatic activity, both groups gave urease reactions and were beta glucosidase-positive. Nevertheless, complete inhibition of the capacity to hydrolyse starch was observed only on the isolates preserved in water. This group also was more sensitive to potassium iodide at a concentration of 10 microM in the in vitro sensitivity tests.
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Bases de datos:
MEDLINE
Asunto principal:
Preservación Biológica
/
Sporothrix
/
Micología
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Idioma:
Es
Revista:
Rev Iberoam Micol
Asunto de la revista:
MICROBIOLOGIA
Año:
2005
Tipo del documento:
Article
País de afiliación:
Venezuela