Clonal dissemination of Escherichia coli O157:H7 subtypes among dairy farms in northeast Ohio.
Appl Environ Microbiol
; 72(4): 2621-6, 2006 Apr.
Article
en En
| MEDLINE
| ID: mdl-16597966
ABSTRACT
To ascertain the extent to which indistinguishable strains of Escherichia coli O157H7 are shared between farms, molecular characterization was performed on E. coli O157H7 isolates recovered during a longitudinal study of 20 dairy farms in northeast Ohio. Of the 20 dairy farms sampled, 16 were located in a primary area and 4 were located in two other distant geographical areas. A total of 92 E. coli O157H7 isolates obtained from bovine fecal samples, water trough sediment samples, free-stall bedding, and wild-bird excreta samples were characterized. Fifty genetic subtypes were observed among the isolates using XbaI and BlnI restriction endonucleases. Most restriction endonuclease digestion profiles (REDPs) were spatially and temporally clustered. However, four REDPs from multiple sources were found to be indistinguishable by pulsed-field gel electrophoresis between four pairs of farms. The geographical distance between farms which shared an indistinguishable E. coli O157H7 REDP ranged from 9 to 50 km, and the on-farm sources sharing indistinguishable REDPs included cattle and wild bird feces and free-stall bedding. Within the study population, E. coli O157H7 REDP subtypes were disseminated with considerable frequency among farms in close geographic proximity, and nonbovine sources may contribute to the transmission of this organism between farms.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Enfermedades de los Bovinos
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Escherichia coli O157
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Industria Lechera
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Infecciones por Escherichia coli
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Crianza de Animales Domésticos
Tipo de estudio:
Observational_studies
/
Risk_factors_studies
Límite:
Animals
País/Región como asunto:
America do norte
Idioma:
En
Revista:
Appl Environ Microbiol
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos