Humanization of yeast to produce complex terminally sialylated glycoproteins.
Science
; 313(5792): 1441-3, 2006 Sep 08.
Article
en En
| MEDLINE
| ID: mdl-16960007
ABSTRACT
Yeast is a widely used recombinant protein expression system. We expanded its utility by engineering the yeast Pichia pastoris to secrete human glycoproteins with fully complex terminally sialylated N-glycans. After the knockout of four genes to eliminate yeast-specific glycosylation, we introduced 14 heterologous genes, allowing us to replicate the sequential steps of human glycosylation. The reported cell lines produce complex glycoproteins with greater than 90% terminal sialylation. Finally, to demonstrate the utility of these yeast strains, functional recombinant erythropoietin was produced.
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Bases de datos:
MEDLINE
Asunto principal:
Pichia
/
Sialoglicoproteínas
/
Ingeniería de Proteínas
/
Eritropoyetina
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Science
Año:
2006
Tipo del documento:
Article
País de afiliación:
Estados Unidos