Fluorescence assay based on aptamer-quantum dot binding to Bacillus thuringiensis spores.
J Fluoresc
; 17(2): 193-9, 2007 Mar.
Article
en En
| MEDLINE
| ID: mdl-17265180
ABSTRACT
A novel assay was developed for the detection of Bacillus thuringiensis (BT) spores. The assay is based on the fluorescence observed after binding an aptamer-quantum dot conjugate to BT spores. The in vitro selection and amplification technique called SELEX (Systematic Evolution of Ligands by EXponential enrichment) was used in order to identify the DNA aptamer sequence specific for BT. The 60 base aptamer was then coupled to fluorescent zinc sulfide-capped, cadmium selenide quantum dots (QD). The assay is semi-quantitative, specific and can detect BT at concentrations of about 1,000 colony forming units/ml.
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Bases de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
/
Bacillus thuringiensis
/
Recuento de Colonia Microbiana
/
Puntos Cuánticos
/
Aptámeros de Nucleótidos
Idioma:
En
Revista:
J Fluoresc
Asunto de la revista:
BIOFISICA
Año:
2007
Tipo del documento:
Article
País de afiliación:
Estados Unidos