Cadmium-induced changes in Per 1 and Per 2 gene expression in rat hypothalamus and anterior pituitary: effect of melatonin.
Toxicol Lett
; 172(3): 131-6, 2007 Aug.
Article
en En
| MEDLINE
| ID: mdl-17590544
ABSTRACT
Chronic cadmium (Cd) administration affects the circadian release of pituitary hormones in rats. To assess whether Cd modifies expression of two major clock genes, period (Per) 1 and Per 2, in the hypothalamic-pituitary unit and to what extent the changes could be prevented by melatonin, rats were exposed to CdCl(2) (5ppm in drinking water) with or without melatonin (3 microg/mL drinking water) for 1 month and were killed at two time intervals, i.e. a the beginning of the rest span (0900h) and at the middle of the activity span (0100h). Hypothalamic and pituitary mRNA levels encoding Per 1 and Per 2 were measured by real-time PCR analysis. Cd treatment decreased expression of hypothalamic Per 1 gene at both time intervals, of hypothalamic Per 2 gene at 0100h, and of adenohypophysial Per 1 and Per 2 genes at 0900h. Melatonin administration counteracted most of the effects of Cd and augmented hypothalamic Per 2, and adenohypophysial Per 1 and Per 2 gene expression. The results indicate that Cd administered chronically in the drinking water to rats affected expression of clock genes in the hypothalamic-pituitary unit, an effect prevented by melatonin.
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Bases de datos:
MEDLINE
Asunto principal:
Adenohipófisis
/
Cadmio
/
Proteínas Nucleares
/
Proteínas de Ciclo Celular
/
Hipotálamo
/
Melatonina
Límite:
Animals
Idioma:
En
Revista:
Toxicol Lett
Año:
2007
Tipo del documento:
Article
País de afiliación:
España