Noncovalent complexes between DNA and basic polypeptides or polyamines by MALDI-TOF.
J Am Soc Mass Spectrom
; 18(11): 1977-89, 2007 Nov.
Article
en En
| MEDLINE
| ID: mdl-17900921
ABSTRACT
MALDI-MS was evaluated as a method for the study of noncovalent complexes involving DNA oligonucleotides and various polybasic compounds (basic polypeptides and polyamines). Complexes involving single-stranded DNA were successfully detected using DHAP matrix in the presence of an ammonium salt. Control experiments confirmed that the interactions involved basic sites of the polybasic compounds and that the complexes were not formed in the gas phase but were pre-existing in the matrix crystals. Moreover, the pre-existence in solution was probed by isothermal titration calorimetry at concentration and ionic strength similar to those used for mass spectrometry. Spectra showed no important difference between negative and positive ion modes. The influence of nature and size of DNA and polybasic compound on the relative intensities and stoichiometries of the complexes was investigated. Despite the fact that relative intensities can be affected by ionization yields and the gas-phase stabilities of the different species, numerous trends observed in the MALDI study were consistent with the expected in-solution behaviors. Experimental conditions related to sample preparation were investigated also. Complex abundance generally decreased when increasing the ammonium acetate concentration. It was dramatically decreased when using ATT instead of DHAP. Penta-L-arginine is an exception to these observations. Lastly, in the case of complexes involving DNA duplex, the ATT matrix was shown to favor the observation of specific DNA duplex but not that of its complex with polybasic compounds. Inversely, DHAP was appropriate for the conservation of DNA-polybasic compound interaction but not for the transfer of intact duplex.
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Bases de datos:
MEDLINE
Asunto principal:
Péptidos
/
ADN
/
Poliaminas Biogénicas
Tipo de estudio:
Evaluation_studies
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Año:
2007
Tipo del documento:
Article
País de afiliación:
Francia