Single-molecule analysis of human telomerase monomer.
Nat Chem Biol
; 4(5): 287-9, 2008 May.
Article
en En
| MEDLINE
| ID: mdl-18391947
ABSTRACT
Human telomerase is a ribonucleoprotein that is minimally comprised of protein (hTERT) and RNA (hTR) components. We have applied single-molecule fluorescence two-color coincidence detection to characterize complex formation between fluorophore-labeled components in solution. By systematic labeling and in vitro assembly of hTERT, hTR and telomerase's DNA substrate, we have established that catalytically functional human telomerase comprises a stable hTERThTRsubstrate interaction in a 111 absolute stoichiometry.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Telomerasa
Límite:
Humans
Idioma:
En
Revista:
Nat Chem Biol
Asunto de la revista:
BIOLOGIA
/
QUIMICA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Reino Unido