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Histochemical localization of the extracellular matrix components in the annular ligament of rat stapediovestibular joint with special reference to fibrillin, 36-kDa microfibril-associated glycoprotein (MAGP-36), and hyaluronic acid.
Ohashi, Mitsuru; Ide, Soyuki; Sawaguchi, Akira; Suganuma, Tatsuo; Kimitsuki, Takashi; Komune, Shizuo.
Afiliación
  • Ohashi M; Department of Anatomy, Ultrastructural Cell Biology, Faculty of Medicine, University of Miyazaki, Miyazaki 889-1692, Japan.
Med Mol Morphol ; 41(1): 28-33, 2008 Mar.
Article en En | MEDLINE | ID: mdl-18470678
The annular ligament across the stapediovestibular joint connects the stapes footplate and the vestibular window and plays an important role in the sound conductive system of the ear. In this study, we investigated the distribution of extracellular matrix components in the ligament by histochemical methods at light and electron microscopic levels. As results, light microscopic immunohistochemistry of fibrillin and 36-kDa microfibril-associated glycoprotein (MAGP-36) showed intense immunoreactivities in the annular ligament between the stapes footplate and vestibular window. In addition, the histochemical localization of hyaluronic acid by using biotinylated hyaluronic acid-binding protein (HABP) clarifi ed the presence of hyaluronic acid in the annular ligament. At the electron microscopic level, the immunogold labeling of fibrillin showed intense labeling on the periphery of the electron-dense mantle. Furthermore, the labeling of fibrillin was preferentially seen on the fibrous components among the electronlucent amorphous substance. The immunogold labeling of MAGP-36 was seen on the electron-dense mantle and scattered on the electron-lucent amorphous substance. The gold labeling with biotinylated HABP clearly showed a distribution of hyaluronic acid throughout the amorphous space in the ligament. The present results provide a histochemical profile of the annular ligament of the rat stapediovestibular joint that may provide clues to elucidation of pathological changes in the ligaments and conductive hearing loss in humans.
Asunto(s)

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Estribo / Vestíbulo del Laberinto / Matriz Extracelular / Articulaciones / Ligamentos Articulares Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Med Mol Morphol Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2008 Tipo del documento: Article País de afiliación: Japón

Texto completo: 1 Bases de datos: MEDLINE Asunto principal: Estribo / Vestíbulo del Laberinto / Matriz Extracelular / Articulaciones / Ligamentos Articulares Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Med Mol Morphol Asunto de la revista: BIOLOGIA MOLECULAR / PATOLOGIA Año: 2008 Tipo del documento: Article País de afiliación: Japón