Marker removal from actinomycetes genome using Flp recombinase.
Gene
; 419(1-2): 43-7, 2008 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-18550297
ABSTRACT
We report here a system for the functional expression of the Flp recombinase in several actinomycetes Streptomyces coelicolor, S. lividans, and Saccharotrix espanaensis. We have constructed a synthetic gene encoding the Flp recombinase with a GC content of 60.6% optimized for expression in high-GC bacteria. Using the synthetic flp(a) gene, we have removed an apramycin resistance gene flanked by FRT sites from the chromosome of actinomycetes with an efficiency of 40%. Sequencing the region of chromosome showed that excision of the apramycin cassette by Flp recombinase was specific.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Actinomycetales
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Farmacorresistencia Microbiana
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Ingeniería Genética
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ADN Nucleotidiltransferasas
Idioma:
En
Revista:
Gene
Año:
2008
Tipo del documento:
Article
País de afiliación:
Alemania