High-sensitivity analysis of glycosphingolipids by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight imaging mass spectrometry on transfer membranes.
J Chromatogr B Analyt Technol Biomed Life Sci
; 870(1): 74-83, 2008 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-18571485
ABSTRACT
Glycosphingolipids are ubiquitous constituents of cells. Yet there is still room for improvement in the techniques for analyzing glycosphingolipids. Here we report our highly sensitive and convenient analytical technology with imaging mass spectrometry for detailed structural analysis of glycosphingolipids. We were able to determine detailed ceramide structures; i.e., both the sphingosine base and fatty acid, by MS/MS/MS analysis on a PVDF membrane with 10 pmol of GM1, with which only faint bands were visible by primuline staining. The limit of detection was approximately 1 pmol of GM1, which is lower than the value in the conventional reports (10 pmol).
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Glicoesfingolípidos
/
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción
Tipo de estudio:
Diagnostic_studies
/
Evaluation_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
J Chromatogr B Analyt Technol Biomed Life Sci
Asunto de la revista:
ENGENHARIA BIOMEDICA
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón