Involvement of cytoskeletal integrity in the regulation of Cl- and amylase secretion from rat parotid acinar cells.
Biomed Res
; 29(3): 131-9, 2008 Jun.
Article
en En
| MEDLINE
| ID: mdl-18614846
ABSTRACT
The cytoskeleton serves as a signal modulator for Ca2+ and cAMP-regulated cell functions including the secretion of ions and granule contents. The interaction between Ca2+ and cAMP signaling systems potentiates amylase secretion and suppresses Cl- secretion in the parotid glands. In this study, we investigated the role of the cytoskeleton in the modulation of Cl- and amylase secretion from rat parotid acinar cells upon activation of each intracellular signaling system and their interaction. Cytochalasin D markedly inhibited the Ca2+-activated outwardly rectifying Cl- current at positive membrane potentials and carbachol (CCh)-induced Cl- currents in the whole-cell configuration at -80 mV, whereas colchicine enhanced Cl- currents. Cytochalasin D, but not colchicine, markedly inhibited CCh-induced Cl- secretion. Synergistic actions of CCh and forskolin on Cl- and amylase secretion were observed even in the presence of cytochalasin D. These results suggest that the synergistic effects of Ca2+ and cAMP signaling systems on amylase and Cl- secretion do not require actin filament integrity but that secretion by the two signals themselves does require actin filament integrity.
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Bases de datos:
MEDLINE
Asunto principal:
Glándula Parótida
/
Citoesqueleto
/
Cloruros
/
Amilasas
Límite:
Animals
Idioma:
En
Revista:
Biomed Res
Año:
2008
Tipo del documento:
Article
País de afiliación:
Japón