Diagnosis of human hepatitis B virus in non-treated blood by the bovine IgG DNA-linked carbon nanotube biosensor.
J Clin Virol
; 44(1): 43-7, 2009 Jan.
Article
en En
| MEDLINE
| ID: mdl-18977688
OBJECTIVES: To identify an effective bioassay for human hepatitis B virus (HBV) using voltammetric sensor. STUDY DESIGN: Electrode was prepared with bovine IgG immobilized onto a DNA-linked carbon nanotube electrode (BIDCE). Analytical BIDCE conditions were optimized through square wave (SW) stripping and cyclic voltammetry (CV). RESULTS: Optimum parameters of 75 Hz SW frequency, 0.1 V SW amplitude, -1.3 V SW accumulation potential, 100 s accumulation time, and 3.0 mV SW increment potential were obtained. Within working ranges of 0.035-0.242 mg/mL anti-bovine IgG, the relative standard deviation of 0.2 mL HBV was observed to be 0.04 (n=4). CONCLUSIONS: Diagnostic application was performed through direct assay of HBV in non-treated human blood.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Sangre
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Técnicas Biosensibles
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Virus de la Hepatitis B
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Hepatitis B
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
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Prognostic_studies
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Clin Virol
Asunto de la revista:
VIROLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Corea del Sur