Transcriptome analysis of Medicago truncatula leaf senescence: similarities and differences in metabolic and transcriptional regulations as compared with Arabidopsis, nodule senescence and nitric oxide signalling.
New Phytol
; 181(3): 563-75, 2009.
Article
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| MEDLINE
| ID: mdl-19021865
ABSTRACT
Here, for the first time, a comprehensive transcriptomics study is presented of leaf senescence in the legume model Medicago truncatula, providing a broad overview of differentially expressed transcripts involved in this process. The cDNA-amplification fragment length polymorphism (AFLP) technique was used to identify > 500 genes, which were cloned and sorted into functional categories according to their gene ontology annotation. Comparison between the datasets of Arabidopsis and M. truncatula leaf senescence reveals common physiological events but differences in the nitrogen metabolism and in transcriptional regulation. In addition, it was observed that a minority of the genes regulated during leaf senescence were equally involved in other processes leading to programmed cell death, such as nodule senescence and nitric oxide signalling. This study provides a wide transcriptional profile for the comprehension of key events of leaf senescence in M. truncatula and highlights a possible regulative role for MADS box transcription factors in the terminal phases of the process.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Senescencia Celular
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Arabidopsis
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Hojas de la Planta
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Perfilación de la Expresión Génica
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Medicago truncatula
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Nódulos de las Raíces de las Plantas
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Óxido Nítrico
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
New Phytol
Asunto de la revista:
BOTANICA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Italia