Evidence of heterogeneity in the antibody response against the platelet antigen 3a; recognition of an 11-mer peptide carrying the HPA-3a polymorphic determinant.
Vox Sang
; 96(3): 252-5, 2009 Apr.
Article
en En
| MEDLINE
| ID: mdl-19159419
ABSTRACT
BACKGROUND:
The immune processes involved in the development of alloantibodies against the human platelet antigens in alloimmune disorders remain unclear. Antibody recognition of the platelet antigens on their respective platelet glycoproteins has been shown to be dependent on glycoprotein conformation. Furthermore, the post-translational modification of glycoproteins adds complexity to the alloantigenic determinants.METHODS:
Nine anti-HPA-3a sera along with several control sera were tested for reactivity to an 11-mer peptide straddling the HPA-3a/b polymorphism. Sera found to specifically recognize the 3a peptide were further assessed by platelet pre-exposure and immunoblotting.RESULTS:
Three of the nine antisera were found to specifically recognize an 11-mer synthetic 3a peptide by ELISA. Further analysis of all anti-HPA-3a sera by Western blot showed that only those reactive to the 3a peptide were able to bind both reduced and non-reduced GPIIb.CONCLUSION:
The results presented in this study provide the first known evidence for the identification of an antibody population capable of recognizing a linear and non-glycosylated form of the HPA-3a epitope.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Péptidos
/
Polimorfismo Genético
/
Enfermedades Autoinmunes
/
Antígenos de Plaqueta Humana
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Isoanticuerpos
Límite:
Female
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Humans
/
Male
Idioma:
En
Revista:
Vox Sang
Año:
2009
Tipo del documento:
Article
País de afiliación:
Australia