Molecular epidemiology of human Blastocystis isolates in France.
Parasitol Res
; 105(2): 413-21, 2009 Aug.
Article
en En
| MEDLINE
| ID: mdl-19290540
ABSTRACT
Blastocystis sp. is the most common eukaryotic parasite in the intestinal tract of humans. Due to its strong impact in public health, in this study, we determined the frequency of different Blastocystis subtypes in patients in France. We hypothesized on the mode of transmission and tested a possible relationship between the subtype and symptomatic status. We obtained a total of 40 stool samples identified as positive for Blastocystis by microscopic examination of smears. Participants consisted of 25 symptomatic and 15 asymptomatic patients, for whom clinical and parasitological data were collected. For nested-polymerase chain reaction and genotyping, DNA was extracted directly from fecal samples or from fecal cultures. Morphological forms observed in fecal cultures were uncorrelated with symptomatic status. Genotyping using partial small subunit rRNA gene analysis identified a total of 43 Blastocystis isolates corresponding to 37 single infections and three mixed infections by two different subtypes. These 43 isolates belonged to five subtypes (1, 2, 3, 4, and 7) with predominance of subtype 3 (53.5%). Patient symptomatic status was uncorrelated with Blastocystis subtype.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Infecciones por Blastocystis
/
Blastocystis
Tipo de estudio:
Screening_studies
Límite:
Adolescent
/
Adult
/
Aged
/
Animals
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Child
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Child, preschool
/
Female
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Humans
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Infant
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Male
País/Región como asunto:
Europa
Idioma:
En
Revista:
Parasitol Res
Asunto de la revista:
PARASITOLOGIA
Año:
2009
Tipo del documento:
Article
País de afiliación:
Francia