Implementation of a novel microarray-based diagnostic test for cancer of unknown primary.
Int J Cancer
; 125(6): 1390-7, 2009 Sep 15.
Article
en En
| MEDLINE
| ID: mdl-19536816
ABSTRACT
Patients with carcinoma of unknown primary (CUP) present with metastatic disease for which the primary site cannot be found, despite extensive standard investigation. Here, we describe the development and implementation of the first clinically available microarray-based test for this cancer type (CUPPrint), based on 633 individual tumors representing 30 carcinoma and 17 noncarcinoma classes. Tissue of origin prediction for either fresh frozen or paraffin-embedded tumor samples is achieved with the use of a custom 8-pack 1.9k microarray and robust classification algorithm. An expression profile of 495 genes was used to predict tumor origin by applying a k-nearest neighbor algorithm. Internal cross-validation and analysis of an independent, previously published, 229-sample dataset revealed that clinically informative predictions were made for up to 94% of samples analyzed. Analysis of 13 previously published CUP specimens yielded predicted tumor origins that supported the clinical suspicion in 12 cases (92%). Microarray profiling presents a promising tool to assist in the identification of the primary tumor and might direct a more tailored treatment for CUP patients.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Neoplasias Primarias Desconocidas
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Análisis de Secuencia por Matrices de Oligonucleótidos
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Perfilación de la Expresión Génica
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Pruebas Diagnósticas de Rutina
Tipo de estudio:
Diagnostic_studies
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Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Int J Cancer
Año:
2009
Tipo del documento:
Article
País de afiliación:
Países Bajos