Dereplication and de novo sequencing of nonribosomal peptides.
Nat Methods
; 6(8): 596-9, 2009 Aug.
Article
en En
| MEDLINE
| ID: mdl-19597502
ABSTRACT
Nonribosomal peptides (NRPs) are of great pharmacological importance, but there is currently no technology for high-throughput NRP 'dereplication' and sequencing. We used multistage mass spectrometry followed by spectral alignment algorithms for sequencing of cyclic NRPs. We also developed an algorithm for comparative NRP dereplication that establishes similarities between newly isolated and previously identified similar but nonidentical NRPs, substantially reducing dereplication efforts.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Péptidos Cíclicos
/
Análisis de Secuencia de Proteína
/
Bases de Datos de Proteínas
/
Biosíntesis de Péptidos Independientes de Ácidos Nucleicos
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2009
Tipo del documento:
Article
País de afiliación:
Estados Unidos