Activation of postsynaptic striatal dopamine receptors, monitored by efflux of cAMP in vivo.
Neuropharmacology
; 29(11): 1011-6, 1990 Nov.
Article
en En
| MEDLINE
| ID: mdl-1982337
ABSTRACT
Intracerebral dialysis was used to monitor the change of extracellular concentration of striatal cAMP in rats anaesthetised with chloral hydrate. Forskolin (1-10 microM), an activator of adenylate cyclase, caused a concentration-dependent increase in efflux of cAMP, which was decreased by (+)PHNO (10 microM), an effect probably mediated by D2 sites, since (-)-sulpiride, a D2 receptor antagonist prevented these effects. Dopamine (1-100 microM) also increased the efflux of cAMP but only when the activity of monoamine oxidase and reuptake of dopamine were concomitantly blocked. The D1 receptor agonist SKF 38393 (1-100 microM) caused a concentration-dependent increase in efflux of cAMP, which was blocked by the D1 receptor antagonist SCH 23390 (1-100 microM), but was unaffected by the D2 receptor antagonist sulpiride (10 microM) or by depletion of the concentration of striatal dopamine after pretreatment with 6-hydroxydopamine. Taken together, these results indicate that intracerebral dialysis may be used to monitor the interaction of drugs with post-synaptic dopamine receptors in vivo.
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Bases de datos:
MEDLINE
Asunto principal:
Colforsina
/
Receptores Dopaminérgicos
/
AMP Cíclico
/
Cuerpo Estriado
Límite:
Animals
Idioma:
En
Revista:
Neuropharmacology
Año:
1990
Tipo del documento:
Article
País de afiliación:
Reino Unido