Deletion of Ogg1 DNA glycosylase results in telomere base damage and length alteration in yeast.
EMBO J
; 29(2): 398-409, 2010 Jan 20.
Article
en En
| MEDLINE
| ID: mdl-19942858
ABSTRACT
Telomeres consist of short guanine-rich repeats. Guanine can be oxidized to 8-oxo-7,8-dihydroguanine (8-oxoG) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG). 8-oxoguanine DNA glycosylase (Ogg1) repairs these oxidative guanine lesions through the base excision repair (BER) pathway. Here we show that in Saccharomyces cerevisiae ablation of Ogg1p leads to an increase in oxidized guanine level in telomeric DNA. The ogg1 deletion (ogg1Delta) strain shows telomere lengthening that is dependent on telomerase and/or Rad52p-mediated homologous recombination. 8-oxoG in telomeric repeats attenuates the binding of the telomere binding protein, Rap1p, to telomeric DNA in vitro. Moreover, the amount of telomere-bound Rap1p and Rif2p is reduced in ogg1Delta strain. These results suggest that oxidized guanines may perturb telomere length equilibrium by attenuating telomere protein complex to function in telomeres, which in turn impedes their regulation of pathways engaged in telomere length maintenance. We propose that Ogg1p is critical in maintaining telomere length homoeostasis through telomere guanine damage repair, and that interfering with telomere length homoeostasis may be one of the mechanism(s) by which oxidative DNA damage inflicts the genome.
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Bases de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
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Telómero
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Eliminación de Gen
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Proteínas de Saccharomyces cerevisiae
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ADN Glicosilasas
Idioma:
En
Revista:
EMBO J
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos