Orientation of the central domains of KSRP and its implications for the interaction with the RNA targets.
Nucleic Acids Res
; 38(15): 5193-205, 2010 Aug.
Article
en En
| MEDLINE
| ID: mdl-20385598
ABSTRACT
KSRP is a multi-domain RNA-binding protein that recruits the exosome-containing mRNA degradation complex to mRNAs coding for cellular proliferation and inflammatory response factors. The selectivity of this mRNA degradation mechanism relies on KSRP recognition of AU-rich elements in the mRNA 3'UTR, that is mediated by KSRP's KH domains. Our structural analysis shows that the inter-domain linker orients the two central KH domains of KSRP-and their RNA-binding surfaces-creating a two-domain unit. We also show that this inter-domain arrangement is important to the interaction with KSRP's RNA targets.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Proteínas de Unión al ARN
/
Regiones no Traducidas 3'
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2010
Tipo del documento:
Article
País de afiliación:
Reino Unido