Transient cold shock enhances zinc-finger nuclease-mediated gene disruption.
Nat Methods
; 7(6): 459-60, 2010 Jun.
Article
en En
| MEDLINE
| ID: mdl-20436476
ABSTRACT
Zinc-finger nucleases (ZFNs) are powerful tools for editing the genomes of cell lines and model organisms. Given the breadth of their potential application, simple methods that increase ZFN activity, thus ensuring genome modification, are highly attractive. Here we show that transient hypothermia generally and robustly increased the level of stable, ZFN-induced gene disruption, thereby providing a simple technique to enhance the experimental efficacy of ZFNs.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Dedos de Zinc
/
Marcación de Gen
/
Desoxirribonucleasas
Límite:
Humans
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos