Targeting of alpha-hemolysin by active or passive immunization decreases severity of USA300 skin infection in a mouse model.
J Infect Dis
; 202(7): 1050-8, 2010 Oct 01.
Article
en En
| MEDLINE
| ID: mdl-20726702
ABSTRACT
Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) infections are predominantly those affecting skin and soft tissues. Although progress has been made, our knowledge of the molecules that contribute to the pathogenesis of CA-MRSA skin infections is incomplete. We tested the hypothesis that alpha-hemolysin (Hla) contributes to the severity of USA300 skin infections in mice and determined whether vaccination against Hla reduces disease severity. Isogenic hla-negative (Deltahla) strains caused skin lesions in a mouse infection model that were significantly smaller than those caused by wild-type USA300 and Newman strains. Moreover, infection due to wild-type strains produced dermonecrotic skin lesions, whereas there was little or no dermonecrosis in mice infected with Deltahla strains. Passive immunization with Hla-specific antisera or active immunization with a nontoxigenic form of Hla significantly reduced the size of skin lesions caused by USA300 and prevented dermonecrosis. We conclude that Hla is a potential target for therapeutics or vaccines designed to moderate severe S. aureus skin infections.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Staphylococcus aureus
/
Toxinas Bacterianas
/
Infecciones Cutáneas Estafilocócicas
/
Inmunización Pasiva
/
Inmunización
/
Proteínas Hemolisinas
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
J Infect Dis
Año:
2010
Tipo del documento:
Article
País de afiliación:
Estados Unidos