Effect of hydrogen peroxide on potassium currents in inner hair cells isolated from guinea pig cochlea.
Neuroreport
; 21(16): 1045-9, 2010 Nov 17.
Article
en En
| MEDLINE
| ID: mdl-20856150
ABSTRACT
Hydrogen peroxide (H2O2) is a ubiquitous reactive oxygen species that can induce several inner ear disorders. In this study, we recorded the potassium (K) currents in acutely isolated inner hair cells of guinea pig cochlea, and investigated the effects of H2O2. We also observed the morphological changes in inner hair cells induced by H2O2. In the H2O2 solutions, the amplitude of outward K currents (I(K,f) and I(K,s)) clearly decreased after perfusion for approximately 15 min. Despite the decrease in outward currents, small inward currents (I(K,n)) did not show any reduction. H2O2 induced morphological changes in the inner hair cells. All the inner hair cells in the H2O2 solutions showed shrinkage and granularity of the cell body and led to loss of viability. These results showed the vulnerability of inner hair cells to reactive oxygen species-induced inner ear disorders.
Texto completo:
1
Bases de datos:
MEDLINE
Asunto principal:
Canales de Potasio
/
Oxidantes
/
Células Ciliadas Auditivas Internas
/
Peróxido de Hidrógeno
Límite:
Animals
Idioma:
En
Revista:
Neuroreport
Asunto de la revista:
NEUROLOGIA
Año:
2010
Tipo del documento:
Article
País de afiliación:
Japón